Media reports on Tuesday had said Rosmah had been asked to present herself to the MACC headquarters yesterday morning to assist its investigations.
Ms Rosmah was summoned separately for questioning by the Malaysian Anti-Corruption Commission (MACC) in the administrative capital of Putrajaya, her second visit in as many weeks.
"Following the arrest, Rosmah will face several charges under the Anti-Money Laundering, Prevention of Terrorism Financing and Proceeds of Unlawful Activities Act (AMLATFPUAA) 2001".
Investigations into the alleged looting of state fund 1MDB by an audacious global fraud ring have already seen her husband Najib Razak hit with more than two dozen charges including corruption and money laundering.
She made no comment to reporters.
The anti-graft agency did not say if the charges against Ms Rosmah relate to 1MDB.
He has consistently denied wrongdoing and has pleaded not guilty to all the charges.
On Sept 26, Rosmah was called in for a second round of questioning - this time on 1MDB - which lasted nearly 13 hours.
The wife of former Malaysian Prime Minister Najib Razak is being grilled by the anti-graft agency for the third time over a massive graft scandal involving the 1MDB state investment fund. Najib was said to have been taken into the building not through the main entrance.
Her lawyers, K. Kumaraendran and Datuk Geethan Ram Vincent, earlier told reporters that the nature of the charges against Rosmah was still unclear.
Rosmah Mansor, the flamboyant wife of Malaysia's former prime minister, was arrested Wednesday by the country's anti-graft agency over a multi-billion-dollar scandal that helped to bring down the last government.
"I suppose tomorrow makes for a great picture and media circus."
Corruption accusations have dogged Najib for years, and came to a head in 2015, when the Wall Street Journal reported that almost $700 million in 1MDB funds was diverted to his personal bank account.
New Prime Minister Mahathir Mohamad reopened investigations into 1MDB that were suppressed under Najib's rule.